Researchers at University of Tsukuba have achieved high-resolution visualization of cellular organelles, such as nuclei and mitochondria, using an external apodized phase contrast (ExAPC) microscope. By effectively suppressing halo artifacts—false images caused by light diffraction—the technique reveals the tightly regulated and remarkably stable movement, spatial arrangement, and morphology of these organelles. The team also observed unlabeled biomolecular condensate-like structures whose molecular components remain unidentified.
High-resolution label-free imaging reveals stable organelle dynamics and spatial organization
